最近の研究活動

2023. Oct

海洋性シアノバクテリアのKaiタンパク質の解析

シアノバクテリアの概日時計の光入力系の分子遺伝学的解析

シアノバクテリアにおけるc-di-GMPの同定

シアノバクテリアにおけるLOVドメインタンパク質の発現解析


研究業績

Toida, K, Kushida W, Yamamoto H., Yamamoto, K., Ishii, K., Uesaka, K., Kanaly RA., Kutsuna, S., Ihara, K., Fujita, Y., Iwasaki, H., 2023 The GGDEF protein Dgc2 suppresses both motility and biofilm formation in the filamentous cyanobacterium Leptolyngbya boryana Microbiology Spectrum. e0483722. doi: 10.1128/spectrum.04837-22.

Kameda, M., Kanaly, R.A., Harada, M., Aoki, S., Tukada, H., Kutsuna, S. 2022 Quantification of cyanobacterial cyclic di-guanosine monophosphate(c-di-GMP) by liquid chromatography electrospray ionization tandem mass spectrometry Journal of Microbiological Methods. Vol. 196, May 2022, 106468.

Muroya, M., Oshima, H., Kobayashi, S., Miura, A., Miyamura, Y., Shiota, H., Onai, K., Ishiura, M., Manabe, K., and Kutsuna, S. 2021 Circadian clock in Arabidopsis thaliana determines flower opening time early in the morning and dominantly closes early in the afternoon Plant and Cell Physiology. Apr 3;pcab048. doi: 10.1093/pcp/pcab048.

Kobayashi, T., Obana, Y., Kuboi, N., Kitayama, Y., Hayashi, S., Oka, M., Wada, N., Arita, K., Shimizu, T., Sato, M., Kanaly, RA., and Kutsuna S. 2016 Analysis of the Fine-Tuning of Cyanobacterial Circadian Phase by Monochromatic Light and Long-Day Conditions. Plant and Cell Physiology. 57:105-114.

Kondou, Y., Noguchi, K., Kutsuna, S., Kawashima, M., Yoneda, A., Ishibashi, M., Muto, S., Ichikawa, T., Nakazawa, M., Matsui, M., and Manabe, K. 2013 Overexpression of DWARF AND LESION FORMATION 1 (DLE1) causes altered activation of plant defense system in Arabidopsis thaliana. Plant Biotechnology. 30:1-8.

Yasumitsu ,H., Tajima ,H., Isobe ,M., Kutsuna ,S., Kawsar ,SM., Fujii ,Y., Kanaly ,RA., Ozeki ,Y., and Yokota ,E. 2013 Fine bubble mixing (FBM) culture of E. coli: a highly cost-effective middle scale-size culture system. Protein & Peptide Letters. 20:213-217.

Tanaka, H., Kitamura, M., Nakano, Y., Katayama, M., Takahashi, Y., Kondo, T., Manabe, K., Omata, T., and Kutsuna, S. 2012 CmpR is Important for Circadian Phasing and Cell Growth. PLANT & CELL PHYSIOLOGY

Kutsuna, S., Kondo, T., Ikegami, H., Uzumaki, T., Katayama, M., and Ishiura, M. 2007. The circadian clock-related gene pex regulates a negative cis element in the kaiA promoter region.J. Bacteriology

Arita, K., Hashimoto H., Igari K., Akaboshi M., Kutsuna S., Sato M., and Shimizu, T.  2007. Structural and biochemical characterization of a cyanobacterium circadian clock-modifier protein.  J. Biological Chemistry. 282:1128-1135.

Takai, N., Ikeuchi, S., K. Manabe, and S. Kutsuna. 2006. Expression of the circadian-clock-related gene pex in cyanobacteria increases in darkness and is required to delay the clock. J. Biological Rhythms.

Kutsuna, S., Nakahira, Y., Katayama, M., M. Ishiura, and T. Kondo. 2005. Transcriptional regulation of the circadian clock operon kaiBC by upstream regions in cyanobacteria. Molecular Microbiology. 57:1474-1484. [Pub Med]

Nakahira, Y., Katayama, M., Miyashita, H., Kutsuna, S., Iwasaki, H., Y. Oyama, and T. Kondo. 2004. Global gene repression by KaiC as a master process of prokaryotic circadian system. Proc Natl Sci Acad USA. 101:881-885. [Pub Med]

Kondou, Y., Mogami, N., Hoshi, F., Kutsuna, S., Nakazawa, M., Sakurai, T., Matsui, M., Kaneko, T., Tabata, S., I. Tanaka, and K. Manabe. 2002. Bipolar localization of putative photoreceptor protein for phototaxis in thermophilic cyanobacterium Synechococcus elongatus. Plant Cell Physiology. 43:1585-1588. [PubMed]

Ishiura, M., S. Kutsuna, S. Aoki, H. Iwasaki, C.R. Andersson, A. Tanabe, S.S. Golden, C.H. Johnson, and T. Kondo. 1998. Expression of a gene cluster kaiABC as a circadian feedback process in cyanobacteria. Science 281:1519-1523.

Kutsuna, S., T. Kondo, S. Aoki, and M. Ishiura. 1998. A period-extender gene, pex, that extends the period of the circadian clock in the cyanobacterium Synechococcus sp. strain PCC 7942. J. Bacteriol. 180:2167-2174.

Kondo, T., N.F. Tsinoremas, S.S. Golden, C.H. Johnson, S. Kutsuna, and M. Ishiura. 1994. Circadian clock mutants of cyanobacteria. Science 266:1233-1236.